Darby Holman
University of Cincinnati
RaMP
This semester I had the opportunity to take part in research at the Cincinnati Children's Hospital through the Biomedical Research & Mentoring Program (RaMP). I was placed in the Gross Lab in the Neurology division, and worked under Christina Gross, PhD and Emma Parkins, a PhD student. I not only learned about various lab techniques, but I also got to experience the research process & learn about life as a PhD candidate. Although my time here was cut short, you can view my final presentation below.
WHAT?
This lab’s overall goal is to understand molecular mechanisms underlying epilepsy and autism spectrum disorders. The project that I was working on was investigating the expression of Kv4.2 proteins over development. We knew that this potassium channel is regulated by a microRNA called miR-324-5p & that this miRNA also contributes to seizure severity. By determining the developmental concentration of this, we can try to determine seizure risk factors. My role was to determine the protein concentration of the samples. First I would verify the genotype, then I would prepare a Bradford Assay/ Western Blot and finally I would analyze the results to get kv4.2 expression.
SO WHAT?
Academically, this has allowed me to put my critical thinking skills to the test and to utilize my classroom knowledge and apply it to a real world situation. Professionally, working under a PhD student has allowed me to see what their education actually looks like which has been fascinating! I found it really beneficial to see what these candidates do on a day to day basis and I was able to connect with other students in the lab, like an MD/PhD student who went through medical school already. Although I am still unsure of what the future holds for me, I now know more about this career path than I ever did before. Personally, I learned a lot about myself and how I work. I was able to see that I enjoy the freedom of working alone and that this kind of work really does interest me. I also realized that asking questions is okay, failure is okay and that I do not have to be perfect at everything the first time it happens.
NOW WHAT?
As stated before, I still don't know what the future has in store for me but I do know now that I enjoy research! Even if I stay on a medical school/ dental school path, I am more inclined to look for a research based school rather than clinical because I enjoy being in the laboratory. I also plan on finding summer research programs or internships next summer based upon this experience and now that I have something under my belt I feel more confident applying to new positions!! Overall, this was a really great experience that I feel so grateful to have had!
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